Infrared spectroscopy and biochemical parameters of rat tissues under heavy metal poisoning conditions

The increasing level of anthropogenic environmental pollution and effective means to reduce the negative impact xenobiotics on animal human health is an urgent problem today. Considering this, purpose study examine effect heavy metals accumulation processes under poisoning conditions, biochemical parameters in body rats. Analogue groups were formed rats same age, gender, weight conduct study. Rats poisoned with solutions copper sulfate, zinc cadmium lead nitrate for 14 days. Using method infrared spectroscopy, substantial differences spatial structure protein components intact animals established. difference between spectral characteristics examined tissues clearly demonstrated by statistical indicators skewness kurtosis. It was determined that copper, zinc, cadmium, ions affects course glycolysis reactions tricarboxylic acid cycle, which leads a likely increase serum concentrations lactate pyruvate, oxaloacetate α-ketoglutarate decrease Malate content compared established conditions poisoning, there also (P < 0.05) blood, liver, kidneys. In metals, pool free amino acids kidneys observed. particular, aspartic acid, valine, glycine, tyrosine, cystine (more than 1.5 times) such decreases; alanine, leucine, serine, taurine, threonine, phenylalanine 2.0 times), lysine – 3.4-4.9 times. Therewith, isoleucine methionine 1.3-1.5 times, ornithine 1.8-2.1 glutamic 4.4-5.3 times experimental group ones identified. results can be helpful professional activities doctors veterinary medicine, toxicologists, biologists, environmentalists used control quality livestock products, toxicological studies, analyse objects